An immunomagnetic
separation (IMS) method was developed for separating Salmonella typhimurium
bacteria from large-volume samples of raw eggs.
An egg was
homogenized with a blender. The homogenized egg material was diluted with DI
water to make a 200 mL sample mixture. Anti-Salmonella
typhimurium antibody coated magnetic micro particles (MMP) were used
to trap the Salmonella bacteria in the sample mixture.
The
Salmonella-trapped MMP were separated from the egg sample matrices by using a
large magnet. An enzyme-linked immunosorbent assay method was adopted and
revised for detecting the bacteria trapped onto the MMP.
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